PUBLICACIONES CIENTÍFICAS

Degradación de enterotoxinas: Osawa R., Matsumoto K. (1997). Digestion of staphylococcal enterotoxin by Bacillus natto. Antonie Van Leeuwenhoek. 71:307-311.

Propiedades anti-virales: Vollenbroich D, Őzel M, Vater J, Kamp R, and Pauli G. (1997). Mechanism of inactivation of enveloped viruses by the biosurfactant surfactin from Bacillus subtilis. Biologicals (25): 289-297.

Protección de los huesos:Tsukamoto Y, Ichise H, Kakuda H, Yamaguchi M. (2000). Intake of fermented soybean (natto) increases circulating vitamin K2 (menaquinone-7) and gamma-carboxylated osteocalcin concentration in normal individuals. J Bone Miner Metab. ;18(4):216-22.

Formación de biofilms: Lombardía E, Rovetto AJ, Arabolaza AL, Grau RR. (2006). A LuxS-dependent cell-to-cell language regulates social behavior and development in Bacillus subtilis. Journal Bacteriology. ;188(12):4442-52.

Propiedades anti-tumorales: Wang C, Ng T, Yuan F, Liu Z, and Liu F. (2007). Induction of apoptosis in human leukemia K562 cells by cyclic lipopeptide from Bacillus subtilis natto T-2. Peptides 28: 1344-1350; doi: 10.1016/j.peptides.2007.06.014.

Mantenimiento de la presión arterial: Kim JY, Gum SN, Paik JK, Lim HH, Kim KC, Ogasawara K, Inoue K, Park S, Jang Y, Lee JH. (2008). Effects of nattokinase on blood pressure: a randomized, controlled trial. Hypertens Res. ;31(8):1583-8.

Propiedades anti-microbianas y anti-tumorales: Cao X-H, Liao Z-Y, Wang C-L, Yang W-Y, and Lu M-F. (2009). Evaluation of a lipopeptide biosurfactant from Bacillus natto TK-1 as a potential source of anti-adhesive, antimicrobial, and antitumor activities. Braz. J. Microbiol.; 40: 373-379.

Propiedades contra la enfermedad de Alzheimer: Hsu RL, Lee KT, Wang JH, Lee LY, Chen RP. (2009). Amyloid-degrading ability of nattokinase from Bacillus subtilis natto. J Agric Food Chem. 28;57(2):503-8.

Propiedades anti-ACV: Murakami K, Yamanaka N, Ohnishi K, Fukayama M, Yoshino M. (2012). Inhibition of angiotensin I converting enzyme by subtilisin NAT (nattokinase) in natto, a Japanese traditional fermented food. Food Funct. ;3(6):674-8.

Propiedades anti-estrés: Nakano M, Yamamoto T, Okamura H, Tsuda A, and Kowatari Y. (2012). Effects of oral supplementation with pyrroloquinoline quinone on stress, fatigue, and sleep. Functional Foods in Health and Disease, 2 (8): 307-324.

Propiedades de multicelularidad: Pedrido ME, de Oña P, Ramirez W, Leñini C, Goñi A, Grau R. (2013). Spo0A links de novo fatty acid synthesis to sporulation and biofilm development in Bacillus subtilis. Molecular Microbiology ;87(2):348-67. 

Propiedades anti-ACV: Ji H, Yu L, Liu K, Yu Z, Zhang Q, Zou F, and Liu B. (2014). Mechanisms of nattokinase in protection of cerebral ischemia. Eur. J. Pharmacol. 745, 144-151; doi: 10.1016/j.ejphar.2014.10.024.

Protección de las bacterias lácticas y contra patógenos: VidyaLaxme B, Rovetto A, Grau R, Agrawal R. (2014). Synergistic effects of probiotic Leuconostoc mesenteroides and Bacillus subtilis in malted ragi (Eleucine corocana) food for antagonistic activity against V. cholerae and other beneficial properties. Journal Food Science Technology 51(11):3072-82.

Dotación genética de Bacillus subtilis natto: Kamada M, Hase S, Sato K, Toyoda A, Fujiyama A, Sakakibara Y. (2014). Whole genome complete resequencing of Bacillus subtilis natto by combining long reads with high-quality short reads. PLoS One. 16;9(10): e109999.

Multicelularidad de Bacillus subtilis natto: Grau RR, de Oña P, Kunert M, Leñini C, Gallegos-Monterrosa R, Mhatre E, Vileta D, Donato V, Hölscher T, Boland W, Kuipers OP, Kovács ÁT. (2015). A Duo of Potassium-Responsive Histidine Kinases Govern the Multicellular Destiny of Bacillus subtilis. mBio. 7;6(4): e00581.

Protección de bacterias lácticas: Yang J, Niu C, and Guo X. (2015). Mixed culture models for predicting intestinal interactions between Escherichia coli and Lactobacillus in the presence of probiotic Bacillus subtilis. Benef. Microbies: 6(6): 871-877; doi: 10.3920/BM2015.0033.

Mantenimiento de niveles fisiológicos de lípidos: Nakano M, Kawasaki Y, Suzuki N, and Takara T. (2015). Effects of pyrroloquinoline quinone disodium salt intake on the serum cholesterol levels of healthy Japanise adults. J. Nutr. Sci. Vitaminol., 61, 233-240.

Propiedades contra la Celiaquía: Wei G, Tian N, Siezen R, Schuppan D, and Helmerhorst E. (2016). Identification of food-grade subtilisins as gluten-degrading enzymes to treat celiac disease. Am. J. Physiol. Gastrointest. Liver Physiol. (311) G571-G580; doi: 10.1152/ajpgi.00185.2016.

Protección del corazón: Nagata C, Wada K, Tamura T, Konishi K, Goto Y, Koda S, Kawachi T, Tsuji M, Nakamura K. (2017). Dietary soy and natto intake and cardiovascular disease mortality in Japanese adults: The Takayama study. Am J Clin Nutr. 105(2):426-431.

Propiedades anti-envejecimiento: Donato V, Ayala FR, Cogliati S, Bauman C, Costa JG, Leñini C, Grau R. (2017). Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway. Nature Communications 30; 8:14332.

Propiedades contra patógenos: Rodriguez Ayala F, Bauman C, Bartolini M, Saball E, Salvarrey M, Leñini C, Cogliati S, Strauch M, Grau R. (2017). Transcriptional regulation of adhesive properties of Bacillus subtilis to extracellular matrix proteins through the fibronectin-binding protein YloA. Molecular Microbiology; 104(5):804-821.

Longevidad larga y saludable: Rodriguez Ayala F, Bauman C, Cogliati S, Leñini C, Bartolini M, Grau R. (2017). Microbial flora, probiotics, Bacillus subtilis and the search for a long and healthy human longevity. Microbial Cell; Volume 4 issue 4. Doi: 10.15698/mic2017.04.569.

Protección contra trombos: Weng Y, Yao J, and Wang K. (2017). Nattokinase: an oral antithrombotic agent for the prevention of cardiovascular disease. Int. J. Mol. Sci.; 18, 523; doi:10.3390(ijms18030523.

Protección de bacterias lácticas: Yahav S, Berkovich Z, Ostrov I, Reifen R, and Shemesh M. (2018). Encapsulation of beneficial probiotic (lactic) bacteria in extracellular matrix from biofilm-forming Bacillus subtilis. Artificial Cells, Nanomedicine Biotechnol.; 46(52): 5974-5982; doi: 10.1080/21691401.2018.1476373.

Protección de bacterias lácticas: Horie M, Koike T, Sugino S, Umeno A, and Yoshida Y. (2018). Evaluation of probiotic and prebiotic-like effects of Bacillus subtilis natto (BN) on growth of lactobacilli. J. Gen. Appl. Microbiol.; 64, 26-33; doi: 10.2323/jgam.2017.03.002.

Propiedades contra los dolores musculares: Sunagawa Y, Okamura N, Miyazaki Y, Shimizu K, Genpei M, Funamoto M, Shimizu S, Katanasaka Y, Morimoto E, Yamakage H, Komiyama M, Satoh-Asahara N, Wada H, Suzuki M, Hasegawa K, Morimoto T. (2018). Effects of products containing Bacillus subtilis var natto on healthy subjects with neck and shoulder stiffness, a double-blind, placebo-controlled, randomized crossover study. Biol Pharm Bull. 41:504–509. doi:10.1248/bpb.b17-00780.

Protección contra hongos: Bartolini M, Cogliati S, Vileta D, Bauman C, Ramirez W, Grau R. (2019). Stress-Responsive Alternative Sigma Factor SigB Plays a Positive Role in the Antifungal Proficiency of Bacillus subtilis. Applied Environmental Microbiology; 85(9): e00178-19.

Protección de la piel: Zhang B, Liu y, Ji   Q, Zhao M, Zeng J, Liu L, Xu X, Yan Y, and Gong A. (2019). Nattokinase crude extract enhances cutaneous wound healing. J. Biomaterial and Tissue Eng., 30;21(1):555. doi: 10.1166/jbt.2017.1697.

Protección ósea: Hu L, Feng J, Wu J, Li W, Gningue S, Yang Z, et al. (2020) Identification of six important amino acid residues of MenA from Bacillus subtilis natto for enzyme activity and formation of menaquinone (Vitamine K2). Enzyme Microb. Technol.; 138:109583. doi: 10.1016/j.enzmictec.2020.109583.

Protección contra la diabetes mellitus: Cardinali N, Bauman C, Rodriguez Ayala F, Grau R. (2020). Two cases of type 2 diabetes mellitus successfully treated with probiotics. Clinical Case Reports 22;8(12):3120-3125. Doi: 10.1002/ccr3.3354.

Protección contra ACV: Pham PT, Han B, Hoang BX. (2020). Nattospores as Effective and Safe Functional Supplements in Management of Stroke. Journal of Medicinal Food. 23(8):879-885.

Propiedades contra la enfermedad de Alzheimer: Cogliati S, Clementi V, Francisco M, Crespo C, Argañaraz F, Grau R.  (2020). Bacillus subtilis Delays Neurodegeneration and Behavioral Impairment in the Alzheimer’s Disease Model Caenorhabditis Elegans. Journal Alzheimer’s Disease.;73(3):1035-1052.

Propiedades neuroprotectoras: Rodriguez Ayala F, Francisco M, Argañaraz F, Crespo C, Clementi V, and Grau R (2021). Healthy aging, neuroprotection and decreased risk of cardiovascular death associated with the consumption of probiotic Bacillus subtilis. Gerontology and Geriatric Studies; Volume 7 Issue 3. doi: 10.31031/GGS.2021.07.000662.

Beneficios sobre la circulación sanguínea: Fujita C, Usui Y, Inoue M. (2022). Effects of Bacillus subtilis Var. Natto products on capillary blood flow in healthy subjects with peripheral coldness: A double-blind, placebo-controlled, randomized parallel study. Food Nutr Sci.:13:211–223.

Propiedades contra la diabetes debida a COVID-19: Rodriguez Ayala F, Cardinali N, and Grau R (2022). Efectiveness of the probiotic Bacillus subtilis DG101 to treat type II diabetes mellitus triggered by SARS-CoV-2 infection. Journal Clinical Images Medical Case Reports; 3(5): 1847. doi: 10.52768/2766-7820/1847

Propiedades contra el sobrepeso y la obesidad: Rodriguez Ayala F, Cardinali N,and Grau R. (2022). Efficient weight loss and type II diabetes control in overweight and obese patients consuming the probiotic Bacillus subtilis DG101: a randomized double-blind placebo-controlled study. Asploro Journal Biomedical Clinical Case Report; 5(1): 51 -58. Doi: 10.36502/2022/ASJBCCR.6263.

Propiedades probióticas: Leñini C, Rodriguez Ayala F, Goñi A, Rateni L, Nakamura A and Grau R (2023). Probiotic properties of Bacillus subtilis DG101 isolated from the traditional Japanize fermented food Nattō. Frontiers in Microbiology. Doi: 10.3389/fmicr.2023.1253480.